Principle:-Potato Dextrose Agar is composed of dehydrated Potato Infusion and Dextrose that encourage luxuriant fungal growth. Agar is added as the solidifying agent. Many standard procedures use a specified amount of sterile tartaric acid (10%) to lower the pH of this medium to 3.5 +/- 0.1, inhibiting bacterial growth. Chloramphenicol acts as a selective agent to inhibit bacterial overgrowth of competing microorganisms from mixed specimens, while permitting the selective isolation of fungi.
Potato infusion | 200 gm |
Dextrose | 20 gm |
Agar | 20 gm |
Distilled water | 1 liter |
And antibiotic can also be used to promot fungal growth and prevent bacterial growth.
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