Friday, July 31, 2020

PDA- POTATO DEXTROSE AGAR MEDIA

Principle:-Potato Dextrose Agar is composed of dehydrated Potato Infusion and Dextrose that encourage luxuriant fungal growth. Agar is added as the solidifying agent. Many standard procedures use a specified amount of sterile tartaric acid (10%) to lower the pH of this medium to 3.5 +/- 0.1, inhibiting bacterial growth. Chloramphenicol acts as a selective agent to inhibit bacterial overgrowth of competing microorganisms from mixed specimens, while permitting the selective isolation of fungi.



Potato infusion200 gm
Dextrose20 gm
Agar20 gm
Distilled water1 liter

Aspergillus flavus and penicilium chrysogenum can be cultivated in PDA medium. 
And antibiotic can also be used to promot fungal growth and prevent bacterial growth. 

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